Publikace detail

Direct infusion mass spectrometry platform for large-scale screening of blood plasma samples

Autoři: Idkowiak Jakub | Jirásko Robert | Wolrab Denise | Holčapek Michal

Rok: 2020

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Direct infusion mass spectrometry platform for large-scale screening of blood plasma samples	Lipidomic analysis has come under the spotlight in clinical research, as lipids are an important group of bioactive metabolites. Lipidomics, which relies strongly on mass spectrometry, guarantees effective characterization of lipid structures with high sensitivity in the framework of commonly applied shotgun lipidomics platforms. The technique exploits a variety of experimental settings offered by the modern mass spectrometry, without time-consuming chromatography. Usually, shotgun lipidomics platforms operate using robotic nanoflow ion sources (e.g. Nanomate device) for sample introduction. However, the beneficial features of the approach are available at relatively high costs, and thereby its application in routine clinical analysis remains limited. In this study, the first results of blood plasma lipidome analysis are presented, with the newly developed flow-injection shotgun lipidomics platform. To minimize analysis costs, regular liquid chromatography pumps were applied to infuse samples into the ion source of the Q-Trap mass spectrometer system. It was found that the approach enables identification and quantitation of a comparable number of lipid species with currently available methodologies.

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