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The preparation of biologically active lactoferrin conjugated with iron oxide nanoparticles and its influence on cytotoxicity of healthy and tumour cell lines

Autoři: Mannová Nikola | Poláková Kateřina | Bílková Zuzana

Rok: 2020

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	The preparation of biologically active lactoferrin conjugated with iron oxide nanoparticles and its influence on cytotoxicity of healthy and tumour cell lines	Effective therapy of tumour diseases is an actual topic worldwide thanks to the fact that cancer remains one of the leading causes of death. Quickly progressing field of nanotechnology brings us new ways of development of effective anti-cancer therapy. This research paper introduces a new concept of therapeutic and diagnostic agent, which combine the known anti-carcinogenic and anti-inflammatory properties of dairy protein lactoferrin and benefits of superparamagnetic iron oxide nanoparticles. The goal of this research is to find out, whether this complex shows anti-carcinogenic properties in vitro or have any other influence on healthy or tumour cell lines. Results obtained by two methods monitoring the cytotoxicity (MTT assay, flow-cytometry) confirmed the biocompatibility of the complex. The viability of healthy and tumour cells didn’t drop below 80% for concentration of SPIO-Lf to 500 µg/ml. Preliminary results of experiments monitoring the oxidative stress denoted no effect and no increase of ROS compared to control cells. We managed to prepare stable and biocompatible complex SPION-Lf, which can be used as contrast agent with high affinity to inflammatory and cancer cells. This agent can be used in targeted diagnostics (for example magnetic resonance imaging) with possible therapeutic effects.	superparamagnetic iron oxide; nanoparticles; lactoferrin; in vitro testing; cell viability

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