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Studying cadmium neurotoxicity in vitro
Rok: 2022
Druh publikace: ostatní - článek ve sborníku
Název zdroje: Military Medical Science Letters
Název nakladatele: Univerzita obrany v Brně
Místo vydání: Brno
Strana od-do: nestránkováno
Tituly:
Jazyk Název Abstrakt Klíčová slova
eng Studying cadmium neurotoxicity in vitro Cadmium is a toxic transition heavy metal commonly found in the environment and its occurrence is mainly conditioned by agricultural and industrial production. Increased exposure to cadmium, especially because of its accumulation in organisms, causes damage of kidney, liver, and bones. Intracellularly, there are proteins, metallothionines, that are actively involved in the detoxification of cadmium by its uptake. In response to increased ROS production after exposure to cadmium, the Nrf2 protein, which modulates glutathione synthesis, is activated (1). The activation rate of Nrf2 protein depends on the concentration of cadmium in a certain time interval (2). In our work, we focused on testing selected concentrations of cadmium chloride (5-100 µM) in SH-SY5Y neuronal cells in vitro. To describe the cellular toxicity, we used a spectrofluorimetric method to determine the intracellular concentration of glutathione (GSH) using monochlorobimane (3) in selected time intervals (1-24 hours). The results suggested that especially at concentrations under 50 µM CdCl2, there is a transient increase in intracellular GSH levels relatively to untreated cells. A significant increase in GSH concentration up to 150% was detected in 5-50 µM CdCl2 treated cells after 24 hours. The financial support was received from Ministry of Education, Youth and Sports of the Czech Republic via projects IT4Neuro (Reg. No. CZ.02.1.01/0.0/0.0/18_069/0010054). Cadmium; Glutathione; Spectrofluorimetry

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