Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Electrochemical Detection of Ergosterol as an Indicator of Fungal Contamination of Foodstuff

Autoři: Korecká Lucie | Kastrati Gylxhane | Martínková Barbora | Brožková Iveta | Mikysek Tomáš | Bílková Zuzana

Rok: 2022

Druh publikace: ostatní - článek ve sborníku

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Electrochemical Detection of Ergosterol as an Indicator of Fungal Contamination of Foodstuff	Ergosterol (provitamine D2, ergosta-5,7,22-trien-3β-ol) is a fungal-specific membrane sterol with structure similar to cholesterol [1,2]. Some fungi are common contaminants of cereals, nuts, dried and other foods, which leads not only to a deterioration of food quality, but especially due to the production of secondary metabolites (mycotoxins), to the risk of health poisoning [3]. In connection to ergosterol, it has been found that there is a correlation between the amount of produced ergosterol and the growth of a given fungus [4,5]. Therefore, ergosterol could be used as biochemical marker of fungal contamination of food raw materials or products. Our aim was to implement and optimize square-wave voltammetric method for detection of ergosterol as simple screening method instead of time-consuming microbiological methods or instrumentally more complex HPLC. Three different working electrodes, concretely glassy carbon electrode (GCE), common boron doped diamond electrode (BDDE) and screen-printed boron doped diamond electrode (SPE-BDDE) were used and compared in terms of calibration range and sensitivity. Conditions of electrochemical detection were optimized. Finally, implemented method was tested for the analysis of the real samples represented by fungal mycelium and grain which confirmed the possibility of ergosterol detection.	Ergosterol; electrochemical detection; Square-wave voltammetry; glassy carbon electrode; boron-dopped diamond electrode; food contaminant