Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Characterization of retention with ternary mobile phases in HILIC chromatography

Autoři: Mrvková Sára | Tošovská Barbora | Česla Petr

Rok: 2024

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Characterization of retention with ternary mobile phases in HILIC chromatography	Recently, the interest in Hydrophilic Interaction Liquid Chromatography (HILIC) applications has been growing significantly, which. It is caused by the increased need for the separation of highly polar and ionized substances, which are difficult to achieve in other modes of liquid chromatography. HILIC is popularly used for the separation of polar compounds and is widely applied in the fields of pharmacy, proteomics, metabolomics, food analysis, bioanalysis or environmental analysis. The aim of this work was to evaluate the potential influence of ternary mobile phases on the retention and selectivity of selected polar substances. The combination of acetonitrile, methanol, and aqueous buffer can influence the character of the diffuse aqueous layer on the surface of the HILIC stationary phases and affect the quality of the separation. Analysis of experiments on different columns confirmed that the ZIC HILIC column is the most suitable for the separation of polar compounds due to its ability to retain these substances and thus provide higher separation efficiency. Subsequent testing of the gradient with ternary mobile phases showed the possibility of using a combination of acetonitrile and methanol in the gradient for the targeted setting of separation selectivity when working with polar compounds.