Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Toxicity of Heavy Metals Tested in a 3D Model

Autoři: Handl Jiří | Tichá Lenka | Vašíčková Veronika | Čapek Jan | Roušar Tomáš

Rok: 2025

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Toxicity of Heavy Metals Tested in a 3D Model	The study of toxicity mechanism of cadmium and other heavy metals is still very relevant because of their constant presence in the environment. Heavy metals induce hepato-, nephro-, and neurotoxic effects. Cadmium induces toxic effect in the central and peripheral nervous systems and in blood‑brain barrier [1]. Current evidence indicates that Cd can be also an etiological factor of neurodegenerative diseases. The human neuroblastoma cell line SH‑SY5Y has been accepted as a suitable in vitro model for the evaluation of CdCl2 neurotoxicity in the literature [2, 3]. At present, the preferred culture model is to use scaffolds, modified culture plastics, or hydrogels, ensuring the formation of 3D models as spheroids and organoids. The aim of our study was to create reproducible spheroids of neuroblastoma cells that will be useful for studying cytotoxicity of heavy metals. First of all, the formation of SH‑SY5Y spheroids was optimized during 7 days of culture. Secondly, the number, size, and morphology of the formed spheroids were characterized and visualized using fluorescence microscopy. We compared the biological effects of CdCl2 and HgCl2 in 3D spheroids and 2D monolayer of SH‑SY5Y cells. The cells were treated with 0-100 μM CdCl2 or 0-100 μM HgCl2 for up to 48 h. Changes in dehydrogenase activity and intracellular glutathione levels were detected. After 24 h, 75 μM CdCl2 and 50 μM HgCl2 caused reduction of dehydrogenase activity in both 2D vs 3D models compared to untreated cells. In conclusion, 3D model of SH‑SY5Y cells was created and used for the CdCl2 and HgCl2 cytotoxicity testing. SH‑SY5Y cells grown in 3D spheroids were less sensitive to the CdCl2 and HgCl2 toxicity compared to the cells grown in 2D. The study was supported by The Biomedical Indicators for Personalized Medicine project (BIPOLE), project ID CZ.02. 01. 01/00/23_021/0008439, co‑funded by the European Union and by the FCHT/SGS_2025_004 project.	heavy metals; cadmium; neuroblastoma cell; SH-SY5Y; neurotoxicity