Publikace detail

Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR.

Autoři: Pejchalová Marcela | Vytřasová Jarmila | Brožková Iveta | Červenka Libor | Husková Zuzana

Rok: 2006

Druh publikace: článek v odborném periodiku

Název zdroje: Journal of Food and Nutrition Research

Název nakladatele: Výskumný ústav potravinársky

Místo vydání: Bratislava

Strana od-do: 152-158

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR.	Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR.	Arcobacter cryaerophilus; Arcobacter butzleri; multiplex-PCR; matrice vzorku
eng	Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR.	Sixty nine meat and water samples were examined by m?PCR assay of the enrichment media. Twenty nine (42 %) of samples were contaminated with Arcobacter species. In 17 samples (24,6 %), A. butzleri was confirmed by m?PCR. Direct detection of arcobacters from assay avoided cultivation on agar plates and reduced the time required for detection. Sensitivity of optimised m-PCR method was verified using four different enrichment broths artificially contaminated with pure cultures of Arcobacters. Formula 6 gave the best results from recommended enrichment media. Influence of matrix samples was tested on real samples of chicken, pork and beef, in which suspension of definite density of Arcobacters was added. It was shown, particularly, that a sample with higher fat content has required a longer incubation period in enrichment broth for successful detection by m?PCR assay.	Arcobacter cryaerophilus;Arcobacter butzleri;multiplex-PCR;matrix of samples

Vyhledávání