Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Biosensors based on immobilization of acetylcholinesterase

Autoři: Vorčáková Katarína | Štěpánková Šárka

Rok: 2012

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Biosensors based on immobilization of acetylcholinesterase	Biosensors are analytical devices composed of three parts: bio-recognition element, physico-chemical convertor and detector [1]. Biosensor utilize the sensitivity and selectivity of a bio-receptor attached onto the surface of a physical transducer. The transducer is able to respond to and transform a bio-chemical and/or physic-chemical property into a measurable signal as a result of a bio-recognition event between the bio-receptor and its target analyte [2]. The most important step in the development of an enzyme biosensor is the stable attachment of the enzyme onto the surface of the working electrode. This process is governed by various interactions between enzyme and the electrode material. Strongly affects the performance of the biosensor in the term of sensitivity, stability, response time and reproducibility. Various immobilization strategies capable of depositing cholinesterases onto the working electrode surface have been used [2]. Types of immobilization are physical adsorption, covalent coupling, self-assembled monolayer, physical entrapment etc. [2, 3]. The aim of this work was compare the different immobilization techniques with using the different working electrodes. We used physical adsorption and crosslinking with glutaraldehyde and four types of screen-printed electrodes. The second part of our work was to investigate the stability of immobilized electrodes and compared their efficiency. Different concentrations of substrate were tested to establish concentration range in which measurements were reproducible.	biosensor, immobilization, acetylcholinesterase