Publikace detail

A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes

Autoři: Pešková Iva | Hrubeš Michal | Červenka Libor | Vytřasová Jarmila | Vytřas Karel

Rok: 2007

Druh publikace: kapitola v odborné knize

Název zdroje: Sensing in Electroanalysis Vol. 2

Název nakladatele: Univerzita Pardubice

Místo vydání: Pardubice

Strana od-do: 201-208

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody	Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody
eng	A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes	An amperometric method for samonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0,65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella enteritidis in a sample.	Salmonella enteritidis, carbon paste electrode, amperometric detection, alkaline phosphatase.

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