Publikace detail

Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from meat-Processing Plants by a Culture Technique and Detection by PCR

Autoři: Vytřasová Jarmila | Pejchalová Marcela | Harsová Klára | Bínová Šárka

Rok: 2003

Druh publikace: článek v odborném periodiku

Název zdroje: Folia Microbiologica

Název nakladatele: Springer

Strana od-do: 227-232

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Izolace Arcobacter butzleri a A. cryaerophilus ze vzorků masa a z masozpracujících závodů kulturními technikamia detekce pomocí PCR	Byla optimalizována technika PCRk detekci jednotlivých druhů arkobakterů . Ze 198 vzorků bylo pozitivních 33 (17%) pro rod Arcobacter a 22(11%) pro A. butzleri.	Arcobacter, masozpracující závod, PCR
eng	Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from meat-Processing Plants by a Culture Technique and Detection by PCR	A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (17%) were found to be positive for genus Arcobacter but only 22 (11%) for A. butzleri.	Arcobacter,Meat-processing, PCR

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