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Výskyt toxinogenních plísní rodu Fusarium v potravinách

Autoři: Brožková Iveta | Vytřasová Jarmila | Svobodová Lucie | Pejchalová Marcela | Červenka Libor

Rok: 2006

Druh publikace: článek ve sborníku

Název zdroje: Sborník Mikrobiologie potravin

Název nakladatele: Vysoká škola chemicko-technologická v Praze

Místo vydání: Praha

Strana od-do: 93-98

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Výskyt toxinogenních plísní rodu Fusarium v potravinách	PCR procedure according to Edwards et al. (2001) was optimised with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F?683 and selectivity was examined using reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non?toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2?3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.
eng	Výskyt toxinogenních plísní rodu Fusarium v potravinách	PCR procedure according to Edwards et al. (2001) was optimised with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F?683 and selectivity was examined using reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non?toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2?3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.	Fusarium, PCR, DNA isolation

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