Publikace detail

Detection of toxigenic fusarium from foods by PCR

Rok: 2007

Druh publikace: článek v odborném periodiku

Název zdroje: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

Název nakladatele: Univerzita Pardubice

Místo vydání: Pardubice

Strana od-do: 73-85

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR	Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR
eng	Detection of toxigenic fusarium from foods by PCR	Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 bp. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphological features are important for species classification where the PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. Thus the PCR technique has proved itself a reliable and rapid method for the detection of the toxigenic species Fusarium.	Fusarium spp.; Fusarium graminearu;, toxigenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins

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